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KMID : 0043320060290111061
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Archives of Pharmacal Research
2006 Volume.29 No. 11 p.1061 ~ p.1065
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Chiral Separation of β-Blockers after Derivatization with a New Chiral Derivatization Agent, GATC
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Ko Mi-Young
Shin Dae-Hong
Oh Joung-Won
Asegahegn Workaferhaw Shibru
Kim Kyeong-Ho
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Abstract
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A new chiral derivatization agent with sugar moiety, 2,3,4,6-tetra-O-acetyl--D-galactopyranosyl isothiocyanate (GATC) was synthesized. Several were investigated for the possible separation of the enantiomers by reversed-phase HPLC after derivatization with this new chiral derivatization agent (GATC). GATC was reacted readily with at room temperature and the reaction mixture could directly be injected into the HPLC system. The corresponding diastereomers were well resolved on an ODS column with acetonitrile-ammonium acetate buffer as a mobile phase and monitored at UV 254 nm. The optimization of the derivatization procedure (concentration of GATC, reaction temperature and time) and HPLC conditions (pH and ionic strength of mobile phase) were investigated and compared with GITC.
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KEYWORD
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Chiral separation, Chiral derivatization agent, HPLC
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